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重要研究成果

陳貴賢 博士
(2020)
Hsiang-Ting Lien, Sun-Tang Chang, Po-Tuan Chen, Deniz P. Wong, Yu-Chung Chang, Ying-Rei Lu, Chung-Li Dong, Chen-Hao Wang, Kuei-Hsien Chen & Li-Chyong Chen 
Nature Commmunictions 11, 4233 (2020).
本研究題目Probing the active site in nonprecious metal-macrocyclic catalysts for ORR via multimodal operando X-ray and electrochemical spectroscopic studies的代表性圖片
Nonnoble metal catalysts are low-cost alternatives to Pt for the oxygen reduction reactions (ORRs), which have been studied for various applications in electrocatalytic systems. Among them, transition metal complexes, characterized by a redox-active single-metal-atom with biomimetic ligands, such as pyrolyzed cobalt–nitrogen–carbon (Co–N x /C), have attracted considerable attention. Therefore, we reported the ORR mechanism of pyrolyzed Vitamin B12 using operando X-ray absorption spectroscopy coupled with electrochemical impedance spectroscopy, which enables operando monitoring of the oxygen binding site on the metal center. material design strategies for high-performance electrocatalysts for fuel cell applications. Furthermore, the charge transfer mechanism between the catalyst and reactant enables further Co–O species formation. These experimental findings, provide insight into metal active-site geometry and structural evolution during ORR, which could be used for developing material design strategies for high performance electrocatalysts for fuel cell applications.
陳貴賢 博士
(2020)
Ming-Chiang Chang, Po-Hsun Ho, Mao-Feng Tseng, Fang-Yuan Lin, Cheng-Hung Hou, I-Kuan Lin, Hsin Wang, Pin-Pin Huang, Chun-Hao Chiang, Yueh-Chiang Yang, I-Ta Wang, He-Yun Du, Cheng-Yen Wen, Jing-Jong Shyue, Chun-Wei Chen, Kuei-Hsien Chen, Po-Wen Chiu & Li-Chyong Chen
Nature Commmunictions 11, 3682 (2020).
本研究題目Self-capping vapor-liquid-solid (VLS) growth of MoS2的代表性圖片
In this study, a self-capping vaporliquid-solid reaction is proposed to fabricate large-grain, continuous MoS2 films. An intermediate liquid phase-Na2Mo2O7 is formed through a eutectic reaction of MoO3 and NaF,followed by being sulfurized into MoS2. The as-formed MoS2 seeds function as a capping layer that reduces the nucleation density and promotes lateral growth. By tuning the driving force of the reaction, large mono/bilayer (1.1 mm/200 μm) flakes or full-coverage films (with a record-high average grain size of 450 μm) can be grown on centimeter-scale substrates. The field-effect transistors fabricated from the full-coverage films show high mobility (33 and 49 cm2V-1s-1 for the mono and bilayer regions) and on/off ratio (1 ~ 5 × 108) across a 1.5 cm × 1.5 cm region.
賴品光 博士
(2020)
Jasper Che-Yung Chien, Elie Tabet, Kelsey Pinkham, Cintia Carla da Hora, Jason Cheng-Yu Chang, Steven Lin, Christian E. Badr* and Charles Pin-Kuang Lai*
Nucleic Acids Research, doi: 10.1093/nar/gkaa669 (2020).
本研究題目A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo的代表性圖片
Tracking DNA double strand break (DSB) repair is paramount for the understanding and therapeutic development of various diseases including cancers. Herein, we describe a multiplexed bioluminescent repair reporter (BLRR) for non-invasive monitoring of DSB repair pathways in living cells and animals. The BLRR approach employs secreted Gaussia and Vargula luciferases to simultaneously detect homology-directed repair (HDR) and non-homologous end joining (NHEJ), respectively. BLRR data are consistent with next-generation sequencing results for reporting HDR (R2 = 0.9722) and NHEJ (R2 = 0.919) events. Moreover, BLRR analysis allows longitudinal tracking of HDR and NHEJ activities in cells, and enables detection of DSB repairs in xenografted tumours in vivo. Using the BLRR system, we observed a significant difference in the efficiency of CRISPR/Cas9-mediated editing with guide RNAs only 1–10 bp apart. Moreover, BLRR analysis detected altered dynamics for DSB repair induced by small-molecule modulators. Finally, we discovered HDR-suppressing functions of anticancer cardiac glycosides in human glioblastomas and glioma cancer stem-like cells via inhibition of DNA repair protein RAD51 homolog 1 (RAD51). The BLRR method provides a highly sensitive platform to simultaneously and longitudinally track HDR and NHEJ dynamics that is sufficiently versatile for elucidating the physiology and therapeutic development of DSB repair.
賴品光 博士
(2020)
Anthony Yan-Tang Wu, Yun-Chieh Sung, Yen-Ju Chen, Steven Ting-Yu Chou, Vanessa Guo, Jasper Che-Yung Chien, John Jun-Sheng Ko, Alan Ling Yang, Hsi-Chien Huang, Ju-Chen Chuang, Syuan Wu, Meng-Ru Ho, Maria Ericsson, Wan-Wan Lin, Chantal Hoi Yin Cheung, Hsueh-Fen Juan, Koji Ueda, Yunching Chen, Charles Pin-Kuang Lai*
Advanced Science, doi:/10.1002/advs.202001467 (2020).
本研究題目Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism的代表性圖片
Extracellular particles (EPs) including extracellular vesicles (EVs) and exomeres play significant roles in diseases and therapeutic applications. However, their spatiotemporal dynamics in vivo have remained largely unresolved in detail due to the lack of a suitable method. Therefore, a bioluminescence resonance energy transfer (BRET)‐based reporter, PalmGRET, is created to enable pan‐EP labeling ranging from exomeres (<50 nm) to small (<200 nm) and medium and large (>200 nm) EVs. PalmGRET emits robust, sustained signals and allows the visualization, tracking, and quantification of the EPs from whole animal to nanoscopic resolutions under different imaging modalities, including bioluminescence, BRET, and fluorescence. Using PalmGRET, it is shown that EPs released by lung metastatic hepatocellular carcinoma (HCC) exhibit lung tropism with varying distributions to other major organs in immunocompetent mice. It is further demonstrated that gene knockdown of lung‐tropic membrane proteins, solute carrier organic anion transporter family member 2A1, alanine aminopeptidase/Cd13, and chloride intracellular channel 1 decreases HCC‐EP distribution to the lungs and yields distinct biodistribution profiles. It is anticipated that EP‐specific imaging, quantitative assays, and detailed in vivo characterization are a starting point for more accurate and comprehensive in vivo models of EP biology and therapeutic design.
汪治平 博士
(2020)
Zan Nie, Chih-Hao Pai, Jie Zhang, Xiaonan Ning, Jianfei Hua, Yunxiao He, Yipeng Wu, Qianqian Su, Shuang Liu, Yue Ma, Zhi Cheng, Wei Lu1, Hsu-Hsin Chu, Jyhpyng Wang, Chaojie Zhang, Warren B. Mori, and Chan Joshi
Nature Communication 11, 2787 (2020)
Availability of relativistically intense, single-cycle, tunable infrared sources will open up newareas of relativistic nonlinear optics of plasmas, impulse IR spectroscopy and pump-probeexperiments in the molecular fingerprint region. However, generation of such pulses is still achallenge by current methods. Recently, it has been proposed that time dependent refractiveindex associated with laser-produced nonlinear wakes in a suitably designed plasma densitystructure rapidly frequency down-converts photons. The longest wavelength photons slipbackwards relative to the evolving laser pulse to form a single-cycle pulse within the nearlyevacuated wake cavity. This process is called photon deceleration. Here, we demonstrate thisscheme for generating high-power (~100 GW), near single-cycle, wavelength tunable(3–20 μm), infrared pulses using an 810 nm drive laser by tuning the density profile of theplasma. We also demonstrate that these pulses can be used to in-situ probe the transient andnonlinear wakes themselves.
 
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