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重要研究成果

陳貴賢 博士
(2019)
Abhishek Pathak, Jing-Wen Shen, Muhammad Usman, Ling-Fang Wei, Shruti Mendiratta, Yu-Shin Chang, Batjargal Sainbileg, Chin-May Ngue, Ruei-San Chen, Michitoshi Hayashi, Tzuoo-Tsair Luo, Fu-Rong Chen, Kuei-Hsien Chen,* Tien-Wen Tseng,* Li-Chyong Chen,* Kuang-Lieh Lu*
Nature Communications 10, Article number: 1721 (2019).
本研究題目Integration of a (–Cu–S–)n plane in a metal–organic framework affords high electrical conductivity的代表性圖片
當前綠能與儲能等研究領域中,設計高導電金屬有機骨架聚合物(MOF)是極具應用潛力的主題。本研究藉由設計銅硫(–Cu–S–)n二維平面於MOF結構之策略來實現高導電MOF之特性。利用水熱法,有機配基6,6’-dithiodinicotinXic acid因原位裂解S–S鍵形成6-mercaptonicotinic acid (1,6-Hmna)與6-mercaptonicotinate (6-mn),並與硝酸銅反應合成MOF化合物{[Cu2(6-Hmna)(6-mn)]·NH4}n。經各種材料分析發現該化合物具(–Cu–S–)n二維平面結構、低活化能(6 meV)、小帶隙(1.34 eV)、及高電導率(10.96 S cm−1)等特性,適用於電池、熱電、超級電容器等相關領域。本研究是由中研院化學所呂光烈、中研院原分所陳貴賢、台大凝態科學中心林麗瓊與台北科大化工系曾添文等教授共同合作,以及台大凝態科學中心林倫年、台灣科大應用科技研究所陳瑞山與清大工程與系統科學系陳福榮等教授協同合作成果,論文已於近期發表於 Nature Communications 期刊  (2019, 10, 1721) 。
余慈顏 博士
(2019)
Yo-Tsen Liu, Yueh-Jung Yen, Frans Ricardo, Yu Chang, Pei-Hao Wu, Shing-Jong Huang, Kon-Ping Lin*,Tsyr-Yan Yu*
Annals of Clinical and Translational Neurology, DOI:10.1002/acn3.50887
本研究題目Biophysical characterization and modulation of Transthyretin Ala97Ser的代表性圖片
Objective: Ala97Ser (A97S) is the major transthyretin (TTR) mutation in Taiwanese patients of familial amyloid polyneuropathy (FAP), characterized by a late-onset but rapidly deteriorated neuropathy. Tafamidis can restore the stability of some mutant TTR tetramers and slow down the progression of TTR-FAP. However, there is little understanding of the biophysical features of A97S-TTR mutant and the pharmacological modulation effect of tafamidis on it. This study aims to delineate the biophysical characteristics of A97S-TTR and the pharmacological modulation effect of tafamidis on this mutant.Method: The stability of TTR tetramers was assessed by urea denaturation and differential scanning calorimetry. Isothermal titration calorimetry (ITC) was used to measure the binding constant of tafamidis to TTR. Nuclear magnetic resonance spectroscopy (NMR) titration experiment was used to map out the tafamidis binding site.Results: Chemical and thermal denaturation confirmed the destabilization effect of A97S. In consistent with other amyloidogenic mutant, A97S-TTR has slightly lower conformational stability. NMR revealed the binding site of A97S-TTR with tafamidis is at the thyroxine binding pocket. The ITC experiments documented the high affinity of the binding which can effectively stabilize the A97S-TTR tetramer.Interpretation: This study confirmed the structural modulation effect of tafamidis on A97S-TTR and implied the potential therapeutic benefit of tafamidis for A97S TTR-FAP. This approach can be applied to investigate the modulation effect of tafamidis on other rare TTR variants and help to make individualized choices of available treatments for FAP patients.
許良彥 博士
(2019)
Siwei Wang, Gregory D. Scholes, and Liang-Yan Hsu*
Journal of Chemical Physics 151, 014105 (2019).
本研究題目Quantum dynamics of a molecular emitter strongly coupled with surface plasmon polaritons: A macroscopic quantum electrodynamics approach [Editor's Pick] [Editor's Choice in 2019]的代表性圖片
We study a molecular emitter above a silver surface in the framework of macroscopic quantum electrodynamics and explore the population dynamics including non-Markovian effects. The theory we present is general for molecular fluorescence in the presence of dielectrics with any space-dependent, frequency-dependent, or complex dielectric functions. Furthermore, the proposed theory allows us to calculate the memory kernel of polaritons using computational electrodynamics packages. In the limit of a high vibration frequency, the different strengths of exciton-polariton couplings lead to distinct characteristics in the population dynamics, e.g., Franck-Condon-Rabi oscillation. (The frequency of Rabi oscillation is dependent on the Franck-Condon factor.) Additionally, in a specific condition, we derive a parameter-free formula that can be used to estimate the exciton-polariton coupling between a molecular emitter and a nanocavity, and the coupling estimated by our theory is in good agreement with the reported experimental results [Chikkaraddy et al., Nature 535, 127–130 (2016)].
高橋開人 博士
(2019)
Daisuke Yoshida*, and Kaito Takahashi*
Journal of Physical Chemistry A, 123, 5300-5308.
本研究題目Odd-Even Reactivity Variation due to Dynamical Effects around the Roaming Saddle Point of the Reaction Between Cn- Chain (n =2-8) and H2的代表性圖片
Unsaturated carbon cluster chains often have chemical properties depending on the cluster size. While carbon cluster cation chains show odd−even variation in the reactivity with hydrogen, thechemistry of the carbon anion chain has been poorly understood even for the bimolecular reaction with hydrogen. We present a systematic theoretical study based on transition state calculations and molecular dynamics trajectory simulations for the reaction of Cn (n = 2−8) + H2. We show that carbon cluster chain anion also has an odd−even variation in reactivity where the even ones are more reactive. In addition, dynamics trajectory shows that while odd n only resulted in the CnH2 product with direct H insertion similar to the static reaction pathway, even n had a more complex product branching producing not only CnH2 but also CnH + H and HCnH with the roaming of an H atom. The flexibility of the carbon’s valence electrons plays an important role to form different isomers of the double H adducts HCnH− and CnH− from the roaming condition.
謝佳龍 博士
(2019)
Yi-Hung Liao, Chih-Hsiang Lin, Ching-Ya Cheng, Wai Cheng (Christine) Wong, Jz-Yuan Juo, Chia-Lung Hsieh*
ACS Nano, Just Accepted (2019).
本研究題目Monovalent and Oriented Labeling of Gold Nanoprobes for the High-Resolution Tracking of a Single Membrane Molecule的代表性圖片
Single-molecule tracking is a powerful method to study molecular dynamics in living systems including biological membranes. High-resolution single-molecule tracking requires a bright and stable signal, which has typically been facilitated by nanoparticles due to their superb optical properties. However, there are concerns about using a nanoparticle to label a single molecule because of its relatively large size and the possibility of crosslinking multiple target molecules, both of which could affect the original molecular dynamics. In this work, using various labeling schemes, we investigate the effects of the use of nanoparticles to measure the diffusion of single membrane molecules. By conjugating a low density of streptavidin (sAv) to gold nanoparticles (AuNPs) of different sizes (10, 15, 20, 30, and 40 nm), we isolate and quantify the effect of the particle size on the diffusion of biotinylated lipids in supported lipid bilayers (SLBs). We find that single sAv tends to crosslink two biotinylated lipids, leading to a much slower diffusion in SLBs. We further demonstrate a simple and robust strategy for the monovalent and oriented labeling of a single lipid molecule with a AuNP by using naturally dimeric rhizavidin (rAv) as a bridge, thus connecting the biotinylated nanoparticle surface and biotinylated target molecule. The rAv-AuNP conjugate demonstrates fast and free diffusion in SLBs (2–3 μm2/s for rAv-AuNP sizes of 10 nm to 40 nm), which is comparable to the diffusion of dye-labeled lipids, indicating that the adverse size and crosslinking effects are successfully avoided. We also note that the diffusion of dye-labeled lipids critically depends on the choice of dye, which could report different diffusion coefficients by about 20 % (2.2 μm2/s of ATTO647N and 2.6 μm2/s of ATTO532). By comparing the diffusion of the uniformly and randomly oriented labeling of a single lipid molecule with a AuNP, we conclude that oriented labeling is favorable for measuring the diffusion of single membrane molecules. Our work shows that the measured diffusion of the membrane molecule is highly sensitive to the molecular design of the crosslinker for labeling. The demonstrated approach of monovalent and oriented AuNP labeling provides the opportunity to study single molecule membrane dynamics at much higher spatiotemporal resolutions, and most importantly, without labeling artifacts.
 
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