研究成果 - 謝佳龍 博士
生物物理與分析技術組
Label-Free Dynamic Imaging of Chromatin in Live Cell Nuclei by High-Speed Scattering-Based Interference Microscopy
ACS Nano, 16(2): 2774-2788 (2022).
Chromatin is a DNA–protein complex that is densely packed in the cell nucleus. The nanoscale chromatin compaction plays critical roles in the modulation of cell nuclear processes. However, little is known about the spatiotemporal dynamics of chromatin compaction states because it remains difficult to quantitatively measure the chromatin compaction level in live cells. Here, we demonstrate a strategy, referenced as DYNAMICS imaging, for mapping chromatin organization in live cell nuclei by analyzing the dynamic scattering signal of molecular fluctuations. Highly sensitive optical interference microscopy, coherent brightfield (COBRI) microscopy, is implemented to detect the linear scattering of unlabeled chromatin at a high speed. A theoretical model is established to determine the local chromatin density from the statistical fluctuation of the measured scattering signal. DYNAMICS imaging allows us to reconstruct a speckle-free nucleus map that is highly correlated to the fluorescence chromatin image. Moreover, together with calibration based on nanoparticle colloids, we show that the DYNAMICS signal is sensitive to the chromatin compaction level at the nanoscale. We confirm the effectiveness of DYNAMICS imaging in detecting the condensation and decondensation of chromatin induced by chemical drug treatments. Importantly, the stable scattering signal supports a continuous observation of the chromatin condensation and decondensation processes for more than 1 h. Using this technique, we detect transient and nanoscopic chromatin condensation events occurring on a time scale of a few seconds. Label-free DYNAMICS imaging offers the opportunity to investigate chromatin conformational dynamics and to explore their significance in various gene activities.
Link to the paper: https://pubs.acs.org/doi/10.1021/acsnano.1c09748
Recommended in Faculty Opinion: https://facultyopinions.com/prime/741388510
Reported by Science Promotion & Engagement Center: https://spec.ntu.edu.tw/20220322-research-chem/
Link to the paper: https://pubs.acs.org/doi/10.1021/acsnano.1c09748
Recommended in Faculty Opinion: https://facultyopinions.com/prime/741388510
Reported by Science Promotion & Engagement Center: https://spec.ntu.edu.tw/20220322-research-chem/
link: 相關連結
目前位置:本所人員 / 研究人員 / 謝佳龍 / 研究成果
中央研究院 原子與分子科學研究所版權所有 |
個人隱私權聲明 |
保有個資檔案公開項目彙整表 |
行動版
地址: 106319 台北市羅斯福路四段一號 或 106923 臺北臺大郵局 第23-166號信箱
電話:886-2-2362-0212 傳真:886-2-2362-0200 電子郵件:iamspublic@gate.sinica.edu.tw
最後更新於 2024-11-01 10:28:55
地址: 106319 台北市羅斯福路四段一號 或 106923 臺北臺大郵局 第23-166號信箱
電話:886-2-2362-0212 傳真:886-2-2362-0200 電子郵件:iamspublic@gate.sinica.edu.tw
最後更新於 2024-11-01 10:28:55