跳到主要內容
 
:::

林靖衛 博士 (生物物理與分析技術組)

林靖衛 博士
辦公室:
338室
辦公室電話:
+886-2-2362-4976
實驗室:
327實驗室
實驗室電話:
+886-2-2366-8253
最新研究成果
Te-I Liu*, Jhih-Shan Wang, Ai-Phuong Nguyen, Marco Raabe, Carlos Jose Quiroz Reyes, Chih-Hsin Lin, Ching-Wei Lin*
ACS Nano 18, 18534–18547 (2024).
Cytometry in the Short-Wave Infrared
Cytometry plays a crucial role in characterizing cell properties, but its restricted optical window (400-850 nm) limits the number of stained fluorophores that can be detected simultaneously and hampers the study and utilization of short-wave infrared (SWIR; 900-1,700 nm) fluorophores in cells. Here we introduce two SWIR-based methods to address these limitations: SWIR flow cytometry and SWIR image cytometry. We develop a quantification protocol for deducing cellular fluorophore mass. Both systems achieve a limit of detection of ~0.1 fg cell−1 within a 30-min experimental timeframe, using individualized, high-purity (6,5) single-wall carbon nanotubes as a model fluorophore and macrophage-like RAW264.7 as a model cell line. This high-sensitivity feature reveals that low-dose (6,5) serves as an antioxidant, and cell morphology and oxidative stress dose-dependently correlate with (6,5) uptake. Our SWIR cytometry holds immediate applicability for existing SWIR fluorophores and offers a solution to the issue of spectral overlapping in conventional cytometry.
瀏覽更多...
 
目前位置:本所人員 / 研究人員/ 林靖衛
回到最上層